The proteomics facility has now reopened. Please contact the new facility manager Marjorie Fournier marjorie.fournier@bioch.ox.ac.uk for any enquiries regarding usage of the facility and discussion of proteomics projects
The Advanced Proteomics Facility provides access to the latest proteomics tools, specifically cutting edge mass spectrometry. It is available to researchers in the South Parks Road science area and to external commercial users.
We have experience in protein identification, quantitative mass spectrometry (SILAC, label-free, dimethyl labelling, TMT) and characterisation methods for a range of post-translational modifications.
The facility is equipped with a wide range of mass spectrometry instrumentation, sample preparation technologies and data analysis software.
Mass Spectrometers and Sample Preparation Equipment
- LTQ XL (tens of proteins identified per hour). Fixed setup for rapid analysis, most likely one sample every 35 to 45 minutes.
- Orbitrap Elite and a number of QExactives (thousands of proteins identified per hour). These instruments are optimised for state of the art analyses. Single analysis will be anything from 45 minutes to 4 hours.
- HPLC systems to allow fractionation of samples for comprehensive proteome coverage.
Bioinformatics Software
- Mascot Server (protein identification).
- Proteome Discover 1.4/2.0 (protein identification and quantitation).
- PEAKS (de novo sequencing and post translational modifications).
- Skyline and Pinpoint (targeted quantitation).
- Progenesis label free quantitation software.
- MaxQuant protein identification and quantification software.
- A number of open source software packages.
Through our service provision and collaboration with researchers, we aim to continually develop and improve the advanced tools available for proteomics.
Location
New Biochemistry building, lab 00-073 and office space 00-068.
Contact and Sample Submission
Please see: https://intranet.bioch.ox.ac.uk/aspsite/services/proteomics - please note that this link will require an Oxford SSO login.
External Work
We have a strong track record of working with biotechnology and pharmaceutical companies. If you are a commercial organisation and are interested in the services or expertise of the Advanced Proteomics Facility, we would encourage you to contact Ms Emma Packard (emma.packard@innovation.ox.ac.uk) of Oxford University Innovation to discuss how we may be able to help you.